analysis of variance (anova) in graphpad prism 5 software  (GraphPad Software Inc)

Journal: Journal of Fungi

Article Title: Differential Physiological Prerequisites and Gene Expression Profiles of Conidial Anastomosis Tube and Germ Tube Formation in Colletotrichum gloeosporioides

doi: 10.3390/jof7070509

Figure Lengend Snippet: Germ tube formation and conidial anastomosis tube fusion dynamics in C. gloeosporioides . ( A ) Percentage GT/CAT formation in different aged conidia, viz., 6, 10, 13, and 17 days of C. gloeosporioides . ( B ) Percentage CAT fusion in 17 days old conidia of C. gloeosporioides incubated for different incubation times, viz., 24, 48, 72, and 96 h. ( C ) Percentage GT formation in 6 days old conidia of C. gloeosporioides incubated for different incubation times, viz., 3, 6, 12, 18, and 24 h. ( D ) Percentage CAT fusion in different conidial densities, viz., 4 × 10 2 , 4 × 10 3 , 4 × 10 4 , 4 × 10 5 , and 4 × 10 6 of 17-day-old conidia incubated in 1 mL of water. ( E ) Percentage GT formation in different conidial densities, viz., 4 × 10 2 , 4 × 10 3 , 4 × 10 4 , 4 × 10 5 , and 4 × 10 6 of 6-day-old conidia incubated in 1 mL of PDB. ( F ) CAT and GT formation percentage in PDB, water, glucose, and Insolution ™ PD98059 (inhibitor of MEK), CAT fusion induced in water supplemented with glucose and Insolution ™ PD98059; GT formation was assayed in PDB supplemented with glucose and Insolution ™ PD98059. GT/CAT formation was quantified as the percentage of conidia involved in GT/CAT formation. Average from three replicates ( n = 3), and 300 conidial numbers were counted per replicate. Bar indicates standard deviation. Statistical significance of differences was analyzed by one-way ANOVA with Tukey’s multiple comparison post-hoc test (bars with the same letter are not significantly different; p ≤ 0.05).

Article Snippet: The GT formation and CAT fusion data were analyzed by Analysis of Variance (ANOVA) with Tukey’s post hoc test using GraphPad Prism 5 Statistics Software.

Techniques: Incubation, Standard Deviation, Comparison

Journal: Frontiers in Immunology

Article Title: Mutations of Recombinant Aquaporin-4 Antibody in the Fc Domain Can Impair Complement-Dependent Cellular Cytotoxicity and Transplacental Transport

doi: 10.3389/fimmu.2018.01599

Figure Lengend Snippet: Antibody Fc domain mutations limit complement-dependent cytotoxicity activation by AQP4–IgG. (A) CHO cells expressing M23–AQP4 were incubated with 5 µg/ml monoclonal antibody in the absence (left row) or presence (right row) of 5% pooled human serum (hC) for 60 min at 37°C. Live cells are labeled with calcein AM, which is a cell viability dye (green); dead cells are labeled with propidium iodide (red). (B) Cell lysis (mean percentage ± SD) is plotted for medium alone, hC alone, and each monoclonal antibody without (top) or with (bottom) hC. Ctl-IgG is specific for measles virus nucleocapsid protein and serves as a negative control. * p < 0.0001; analysis of variance with Dunnett’s multiple comparisons test.

Article Snippet: Statistical analysis was performed using analysis of variance (ANOVA) and Dunnett’s test for multiple comparisons in GraphPad Prism 5 software.

Techniques: Activation Assay, Expressing, Incubation, Labeling, Lysis, Virus, Negative Control

Journal: Frontiers in Immunology

Article Title: Mutations of Recombinant Aquaporin-4 Antibody in the Fc Domain Can Impair Complement-Dependent Cellular Cytotoxicity and Transplacental Transport

doi: 10.3389/fimmu.2018.01599

Figure Lengend Snippet: Quantification of transplacental transport of antibodies. Quanitification of optical intensity of the infrared signal (pixel intensity) from embryos exposed to maternal infrared labeled antibodies in utero . The values of each embryo is averaged and shown normalized to the average pixel intensity of an AQP4–IgG exposed litter present on the same scan to account for variability within scans (normalized pixel intensity). Transplacental transport of each antibody is shown for both litters. One-Way analysis of variance followed by Dunnett’s multiple comparisons test revealed that mice injected with PG-IgG, KA-IgG, and ND-IgG showed markedly reduced normalized infrared pixel intensity compared to AQP4–IgG (** p < 0.0001). DA-IgG showed a modest, but significant reduction, infrared pixel intensity compared to AQP4–IgG (* p < 0.005). Ctl-IgG, AQP4-IgG, and PA-IgG are not different ( p > 0.8).

Article Snippet: Statistical analysis was performed using analysis of variance (ANOVA) and Dunnett’s test for multiple comparisons in GraphPad Prism 5 software.

Techniques: Labeling, In Utero, Injection